Octopus (<i>Enterocopus dofleini</i>) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells

Yasushi Hasegawa; Tomohiko Hori

doi:doi:10.11648/j.aff.20170606.15

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Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells

Yasushi Hasegawa, Tomohiko Hori

Published in Agriculture, Forestry and Fisheries (Volume 6, Issue 6)

Received: 11 October 2017 Accepted: 26 October 2017 Published: 22 November 2017

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Abstract

Fatty liver disease is characterized by the accumulation of triglycerides and other fats in the liver cells and is believed to be a risk of later chronic liver disease. Diet is one of the key ways to treat fatty liver disease. Octopus (Enterocopus dofleini) liver is eaten in some regions in Japan, but mostly discarded. For utilization of octopus liver, the lipid-lowering effect of octopus (Enterocopus dofleini) liver extract was investigated using human hepatoma cells (HepG2 cells). The present study showed that the octopus liver extract reduced the triglyceride content, but not cholesterol content, in HepG2 cells. Treatment with the octopus liver extract increased the mRNA expression of genes for peroxisome proliferator-activated receptor (PPAR)-α, medium chain acyl-CoA dehydrogenase (MCAD), and acyl-CoA oxidase (ACO) associated with β-oxidation. On the contrary, the extract did not change the mRNA expression of genes for sterol regulatory element-binding protein (SREBP)-1, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, and peroxisome proliferator-activated receptor (PPAR)-γ involved in fatty acid synthesis. These results suggest that octopus liver extract may decrease the triglyceride level in HepG2 cells by promoting β-oxidation, suggestive of its usefulness as a food for lowering triglycerides in liver.

Published in	Agriculture, Forestry and Fisheries (Volume 6, Issue 6)
DOI	10.11648/j.aff.20170606.15
Page(s)	214-218
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Efficient Utilization, Octopus Liver Extract, HepG2 Cells, Triglyceride

References

[1]	Lewis JR, Mohanty SR. Nonalcoholic fatty liver disease: a review and update. Digestive Diseases and Sciences, 55, 2010, 560-578.
[2]	Reddy JK, Sambasiva Rao M. Lipid Metabolism and Liver Inflammation. II. Fatty liver disease and fatty acid oxidation. American Journal of Physiology Gastrointestinal and Liver Physiology, 290, 2006, G852 G858.
[3]	Benedict M, Zhang X. Non-alcoholic fatty liver disease: An expanded review. World Journal of Hepatology, 9, 2017, 715-732.
[4]	Mantena, SK, King AL, Andringa KK, Eccleston HB, Bailey SM. Mitochondrial dysfunction and oxidative stress in the pathogenesis of alcohol- and obesity-induced fatty liver diseases. Free Radical Biology & Medicine, 44, 2008, 1259-1272.
[5]	Parker HM, Johnson NA, Burdon CA, Cohn JS, O’Connor HT, George J. Omega-3 supplementation and non-alcoholic fatty liver disease: A systematic review and meta-analysis. Journal of Hepatology, 56, 2012, 944-951.
[6]	Jump DB, Lytle KA, Depner CM, Tripathy S. Omega-3 polyunsaturated fatty acids as a treatment strategy for nonalcoholic fatty liver disease. Pharmacology and Therapeatics, 16, 2017, in press.
[7]	Birerdinc A, Stepanova M, Pawloski L, Younossi ZM. Caffeine is protective in patients with non-alcoholic fatty liver disease. Alimentary Pharmacology & Terapeutics 35, 2011, 76-82.
[8]	Sakata R, Nakamura T, Torimura T, Ueno T, Sata M. Green tea with high-density catechins improves liver function and fat infiltration in non-alcoholic fatty liver disease (NAFLD) patients: A double-blind placebo-controlled study. International Journal of Molecular Medicine 32, 2013, 989-994.
[9]	Manthorpe M, Fagnani R, Skaper SD, Varon S. An automated colorimetric microassay for neuronotrophic factors. Brain Research, 390, 1986, 191-198.
[10]	Granot E, Schwiegelshohn B, Tabas I, Gorecki M, Vogel T, Carpentier YA, Deckelbaum RJ. Effects of particle size on cell uptake of model triglyceride-rich particles with and without apoprotein E. Biochemistry, 33, 1994, 15190-15197.
[11]	Kim SM, Lee B, An HJ, Kim DH, Park KC, Noh SG, Chung KW, Lee EK, Kim KM, Kim DH, Kim SJ, Chun P, Lee HJ, Moon HR, Chung HY Novel PPARα agonist MHY553 alleviates hepatic steatosis by increasing fatty acid oxidation and decreasing inflammation during aging. Oncotarget, 8, 2017, 46273-46285.
[12]	Crabb DW, Galli A, Fischer M, You M. Molecular mechanisms of alcoholic fatty liver: role of peroxisome proliferator-activated receptor alpha. Alcohol, 34, 2004, 35-38.
[13]	Felix AD, Takahashi N, Takahashi M, Katsumata-Tsuboi R, Satoh R, Soon Hui T, Miyajima K, Nakae D, Inoue H, Uehara M. Extracts of black and brown rice powders improve hepatic lipid accumulation via the activation of PPAR in obese and diabetic model mice. Bioscience, Biotechnology, and Biochemistry, 22, 2017, 1-3.
[14]	Feng X, Yu W, Li X, Zhou F, Zhang W Shen Q, Li J, Zhang C, Shen P. Apigenin, a modulator of PPARγ, attenuates HFD-induced NAFLD by regulating hepatocyte lipid metabolism and oxidative stress via Nrf2 activation. Biochemical Pharmacology, 136, 2017, 136-149.
[15]	Wang LF, Wang XN, Huang CC, Hu L, Xiao YF, Guan XH, Qian YS, Deng KY, Xin HB. Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1/AMPKα/SREBP1 signaling pathway. Lipids in Health & Disease, 16, 2017, 82.
[16]	Radler U, Stangl H, Lechner S, Lienbacher G, Krepp R, Zeller E, Brachinger M, Eller-Berndl D, Fischer A, Anzur C, Schoerg G, Mascher D, Laschan C, Anderwald C, Lohninger A. A combination of (ω-3) polyunsaturated fatty acids, polyphenols and L-carnitine reduces the plasma lipid levels and increases the expression of genes involved in fatty acid oxidation in human peripheral blood mononuclear cells and HepG2 cells. Annals of Nutrition and Metabolism, 58, 2011, 133-140.
[17]	Shimoda H, Tanaka J., Kikuchi M., Fukuda T., Ito H, Hatano T, Yoshida T. Effect of polyphenol-rich extract from walnut on diet-induced hypertriglyceridemia in mice via enhancement of fatty acid oxidation in the liver. Journal of Agricultural and Food Chemistry, 57, 2009, 1786-1792.
[18]	Wang S, Huang Y, Xu H, Zhu Q, Lu H, Zhang M, Hao S, FangC, Zhang D, Wu X, Wang X, Sheng J. Oxidized tea polyphenols prevents lipid accumulation in liver and visceral white adipose tissue in rats. European Journal of Nutrition, 56, 2017, 2037-2048.
[19]	Mikami N, Hosokawa M, Miyashita K. Dietary combination of fish oil and taurine decreases fat accumulation and ameliorates blood glucose levels in type 2 diabetic/obese KK-A(y) mice. Journal of Food Science, 77, 2012, H114-H120.

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APA Style

Yasushi Hasegawa, Tomohiko Hori. (2017). Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agriculture, Forestry and Fisheries, 6(6), 214-218. https://doi.org/10.11648/j.aff.20170606.15

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ACS Style

Yasushi Hasegawa; Tomohiko Hori. Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agric. For. Fish. 2017, 6(6), 214-218. doi: 10.11648/j.aff.20170606.15

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AMA Style

Yasushi Hasegawa, Tomohiko Hori. Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agric For Fish. 2017;6(6):214-218. doi: 10.11648/j.aff.20170606.15

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@article{10.11648/j.aff.20170606.15,
  author = {Yasushi Hasegawa and Tomohiko Hori},
  title = {Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {6},
  number = {6},
  pages = {214-218},
  doi = {10.11648/j.aff.20170606.15},
  url = {https://doi.org/10.11648/j.aff.20170606.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20170606.15},
  abstract = {Fatty liver disease is characterized by the accumulation of triglycerides and other fats in the liver cells and is believed to be a risk of later chronic liver disease. Diet is one of the key ways to treat fatty liver disease. Octopus (Enterocopus dofleini) liver is eaten in some regions in Japan, but mostly discarded. For utilization of octopus liver, the lipid-lowering effect of octopus (Enterocopus dofleini) liver extract was investigated using human hepatoma cells (HepG2 cells). The present study showed that the octopus liver extract reduced the triglyceride content, but not cholesterol content, in HepG2 cells. Treatment with the octopus liver extract increased the mRNA expression of genes for peroxisome proliferator-activated receptor (PPAR)-α, medium chain acyl-CoA dehydrogenase (MCAD), and acyl-CoA oxidase (ACO) associated with β-oxidation. On the contrary, the extract did not change the mRNA expression of genes for sterol regulatory element-binding protein (SREBP)-1, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, and peroxisome proliferator-activated receptor (PPAR)-γ involved in fatty acid synthesis. These results suggest that octopus liver extract may decrease the triglyceride level in HepG2 cells by promoting β-oxidation, suggestive of its usefulness as a food for lowering triglycerides in liver.},
 year = {2017}
}

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TY  - JOUR
T1  - Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells
AU  - Yasushi Hasegawa
AU  - Tomohiko Hori
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.aff.20170606.15
DO  - 10.11648/j.aff.20170606.15
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 214
EP  - 218
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20170606.15
AB  - Fatty liver disease is characterized by the accumulation of triglycerides and other fats in the liver cells and is believed to be a risk of later chronic liver disease. Diet is one of the key ways to treat fatty liver disease. Octopus (Enterocopus dofleini) liver is eaten in some regions in Japan, but mostly discarded. For utilization of octopus liver, the lipid-lowering effect of octopus (Enterocopus dofleini) liver extract was investigated using human hepatoma cells (HepG2 cells). The present study showed that the octopus liver extract reduced the triglyceride content, but not cholesterol content, in HepG2 cells. Treatment with the octopus liver extract increased the mRNA expression of genes for peroxisome proliferator-activated receptor (PPAR)-α, medium chain acyl-CoA dehydrogenase (MCAD), and acyl-CoA oxidase (ACO) associated with β-oxidation. On the contrary, the extract did not change the mRNA expression of genes for sterol regulatory element-binding protein (SREBP)-1, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, and peroxisome proliferator-activated receptor (PPAR)-γ involved in fatty acid synthesis. These results suggest that octopus liver extract may decrease the triglyceride level in HepG2 cells by promoting β-oxidation, suggestive of its usefulness as a food for lowering triglycerides in liver.
VL  - 6
IS  - 6
ER  -

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Author Information

Yasushi Hasegawa

Department of Applied Sciences, Muroran Institute of Technology, Hokkaido, Japan
Tomohiko Hori

Department of Applied Sciences, Muroran Institute of Technology, Hokkaido, Japan

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Plain Text BibTeX RIS

APA Style

Yasushi Hasegawa, Tomohiko Hori. (2017). Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agriculture, Forestry and Fisheries, 6(6), 214-218. https://doi.org/10.11648/j.aff.20170606.15

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ACS Style

Yasushi Hasegawa; Tomohiko Hori. Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agric. For. Fish. 2017, 6(6), 214-218. doi: 10.11648/j.aff.20170606.15

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AMA Style

Yasushi Hasegawa, Tomohiko Hori. Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells. Agric For Fish. 2017;6(6):214-218. doi: 10.11648/j.aff.20170606.15

Copy | Download

@article{10.11648/j.aff.20170606.15,
  author = {Yasushi Hasegawa and Tomohiko Hori},
  title = {Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {6},
  number = {6},
  pages = {214-218},
  doi = {10.11648/j.aff.20170606.15},
  url = {https://doi.org/10.11648/j.aff.20170606.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20170606.15},
  abstract = {Fatty liver disease is characterized by the accumulation of triglycerides and other fats in the liver cells and is believed to be a risk of later chronic liver disease. Diet is one of the key ways to treat fatty liver disease. Octopus (Enterocopus dofleini) liver is eaten in some regions in Japan, but mostly discarded. For utilization of octopus liver, the lipid-lowering effect of octopus (Enterocopus dofleini) liver extract was investigated using human hepatoma cells (HepG2 cells). The present study showed that the octopus liver extract reduced the triglyceride content, but not cholesterol content, in HepG2 cells. Treatment with the octopus liver extract increased the mRNA expression of genes for peroxisome proliferator-activated receptor (PPAR)-α, medium chain acyl-CoA dehydrogenase (MCAD), and acyl-CoA oxidase (ACO) associated with β-oxidation. On the contrary, the extract did not change the mRNA expression of genes for sterol regulatory element-binding protein (SREBP)-1, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, and peroxisome proliferator-activated receptor (PPAR)-γ involved in fatty acid synthesis. These results suggest that octopus liver extract may decrease the triglyceride level in HepG2 cells by promoting β-oxidation, suggestive of its usefulness as a food for lowering triglycerides in liver.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Octopus (Enterocopus dofleini) Liver Extract Displays Triglyceride-Lowering Effect in HepG2 Cells
AU  - Yasushi Hasegawa
AU  - Tomohiko Hori
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.aff.20170606.15
DO  - 10.11648/j.aff.20170606.15
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 214
EP  - 218
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20170606.15
AB  - Fatty liver disease is characterized by the accumulation of triglycerides and other fats in the liver cells and is believed to be a risk of later chronic liver disease. Diet is one of the key ways to treat fatty liver disease. Octopus (Enterocopus dofleini) liver is eaten in some regions in Japan, but mostly discarded. For utilization of octopus liver, the lipid-lowering effect of octopus (Enterocopus dofleini) liver extract was investigated using human hepatoma cells (HepG2 cells). The present study showed that the octopus liver extract reduced the triglyceride content, but not cholesterol content, in HepG2 cells. Treatment with the octopus liver extract increased the mRNA expression of genes for peroxisome proliferator-activated receptor (PPAR)-α, medium chain acyl-CoA dehydrogenase (MCAD), and acyl-CoA oxidase (ACO) associated with β-oxidation. On the contrary, the extract did not change the mRNA expression of genes for sterol regulatory element-binding protein (SREBP)-1, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, and peroxisome proliferator-activated receptor (PPAR)-γ involved in fatty acid synthesis. These results suggest that octopus liver extract may decrease the triglyceride level in HepG2 cells by promoting β-oxidation, suggestive of its usefulness as a food for lowering triglycerides in liver.
VL  - 6
IS  - 6
ER  -

Copy | Download