Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis

Yuko Niidome-Matsuda; Junko Hatakeyama; Masaaki Takezaki; Masahiro Yoneda; Yuji Hatakeyama; Sachio Tamaoki

doi:doi:10.11648/j.ajbio.20221001.15

| Peer-Reviewed

Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis

Yuko Niidome-Matsuda, Junko Hatakeyama, Masaaki Takezaki, Masahiro Yoneda, Yuji Hatakeyama, Sachio Tamaoki

Published in American Journal of BioScience (Volume 10, Issue 1)

Received: 6 December 2021 Accepted: 5 January 2022 Published: 18 January 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The synchondroses in the cranial base are important structures in craniofacial growth, and the spheno-occipital synchondrosis (SOS) is representative of a typical growth site. Endoplasmic reticulum (ER) stress is associated with multiple biological processes and is a critical factor in chondrogenesis. It has been reported that 78kDa Glucose-regulated protein (Grp78) plays an important role in suppressing regulators in ER stress-mediated apoptosis in chondrogenesis, and Cripto is the cell surface signaling partner of Grp78 in the transforming growth factor-β (TGF-β) signaling pathway. We attempt to clarify the immunolocalization of Grp78 and Cripto in the SOS. The mice head at embryonic day 17.5 (E17.5) were collected and embedded in paraffin. The serial sections were stained with hematoxylin and eosin, Alcian blue, lectin, and immunostaining. The SOS structure containing the resting, proliferative, and hypertrophic zone were identified with Alcian blue staining, wheat germ agglutinin, and Type II Collagen immunostaining. Immunostaining of Grp78 in the SOS revealed positive immunoreactivity in all the chondrocytes of the SOS. However, the chondrocytes of the proliferating zone were weakly immunopositive to Cripto, while the chondrocytes of the hypertrophic zone were strongly immunopositive. Since the immunolocalization of Grp78 and Cripto was different in cartilage zone, these data suggest that Grp78 and Cripto would be involved in the regulation of hypertrophic chondrocyte differentiation and may be related with ER stress in the SOS.

Published in	American Journal of BioScience (Volume 10, Issue 1)
DOI	10.11648/j.ajbio.20221001.15
Page(s)	31-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Spheno-occipital Synchondrosis (SOS), Grp78, Cripto

References

[1]	Bassed RB, Briggs C, Drummer OH. (2010) Analysis of time of closure of the spheno-occipital synchondrosis using computed tomography. Forensic. Sci. Int. 200: 161.
[2]	McGrath J, Gerety PA, Derderian CA, Steinbacher DM, Vossough A, Bartlett SP, Nah HD, Taylor JA. (2012) Differential closure of the spheno-occipital synchondrosis in syndromic craniosynostosis. Plast Reconstr Surg. 130 (5): 681e-689e.
[3]	Tahiri Y, Paliga JT, Vossough A, Bartlett SP, Taylor JA. (2014) The spheno-occipital synchondrosis fuses prematurely in patients with Crouzon syndrome and midface hypoplasia compared with age- and gender-matched controls. J Oral Maxillofac Surg. 72 (6): 1173-9.
[4]	Rao RV, Peel A, Logvinova A, del Rio G, Hermel E, Yokota T, Goldsmith PC, Ellerby LM, Ellerby HM, Bredesen DE. (2002) Coupling endoplasmic reticulum stress to the cell death program: role of the ER chaperone GRP78. FEBS Lett. 514 (2-3): 122-8.
[5]	Watson LM, Chan AK, Berry LR, Li J, Sood SK, Dickhout JG, Xu L, Werstuck GH, Bajzar L, Klamut HJ, Austin RC. (2003) Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity. J Biol Chem. 278 (19): 17438-47.
[6]	Gorbatyuk MS, Knox T, LaVail MM, Gorbatyuk OS, Noorwez SM, Hauswirth WW, Lin JH, Muzyczka N, Lewin AS. (2010) Restoration of visual function in P23H rhodopsin transgenic rats by gene delivery of BiP/Grp78. Proc Natl Acad Sci U S A. 107 (13): 5961-6.
[7]	Bai Y, Wei Y, Wu L, Wei J, Wang X, Bai Y. (2016) C/EBP β mediates endoplasmic reticulum stress regulated inflammatory response and extracellular matrix degradation in LPS-stimulated human periodontal ligament cells. Int J Mol Sci. 17 (3): 385.
[8]	Saito A, Hino S, Murakami T, Kanemoto S, Kondo S, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Imaizumi K. (2009) Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. Nat Cell Biol. 11 (10): 1197-204.
[9]	De la Fuente A, Mateos J, Lesende-Rodríguez I, Calamia V, Fuentes-Boquete I, de Toro FJ, Arufe MC, Blanco FJ. (2012) Proteome analysis during chondrocyte differentiation in a new chondrogenesis model using human umbilical cord stroma mesenchymal stem cells. Mol Cell Proteomics. 11 (2): M111.010496.
[10]	Xiong Z, Jiang R, Li X, Liu Y, Guo F. (2015) Different roles of GRP78 on cell proliferation and apoptosis in cartilage development. Int J Mol Sci. 16 (9): 21153-76.
[11]	Liu Y, Zhu H, Yan X, Gu H, Gu Z, Liu F. (2017) Endoplasmic reticulum stress participates in the progress of senescence and apoptosis of osteoarthritis chondrocytes. Biochem Biophys Res Commun. 491 (2): 368-373.
[12]	Shani G, Fischer WH, Justice NJ, Kelber JA, Vale W, Gray PC. (2008) GRP78 and Cripto form a complex at the cell surface and collaborate to inhibit transforming growth factor beta signaling and enhance cell growth. Mol Cell Biol. 28 (2): 666-77.
[13]	Yun S, Yun CW, Lee JH, Kim S, Lee SH. (2021) Cripto enhances proliferation and survival of mesenchymal stem cells by up-regulating JAK2/STAT3 pathway in a GRP78-dependent manner. Biomol Ther (Seoul); 29 (4): 452-453.
[14]	Guo FJ, Jiang R, Xiong Z, Xia F, Li M, Chen L, Liu CJ. (2014) IRE1a constitutes a negative feedback loop with BMP2 and acts as a novel mediator in modulating osteogenic differentiation. Cell Death Dis. 5 (5): e1239.
[15]	Han X, Zhou J, Zhang P, Song F, Jiang R, Li M, Xia F, Guo FJ. (2013) IRE1α dissociates with BiP and inhibits ER stress-mediated apoptosis in cartilage development. Cell Signal. 25 (11): 2136-46.
[16]	Gray PC, Vale W. (2012) Cripto/GRP78 modulation of the TGF-β pathway in development and oncogenesis. FEBS Lett. 586 (14): 1836-45.
[17]	Ravindran S, Gao Q, Ramachandran A, Sundivakkam P, Tiruppathi C, George A. (2012) Expression and distribution of grp-78/bip in mineralizing tissues and mesenchymal cells. Histochem Cell Biol. 138 (1): 113-25.
[18]	Kemaladewi DU, de Gorter DJ, Aartsma-Rus A, van Ommen GJ, ten Dijke P,'t Hoen PA, Hoogaars WM. (2012) Cell-type specific regulation of myostatin signaling. FASEB J. 26 (4): 1462-72.
[19]	Garcia de Vinuesa A, Sanchez-Duffhues G, Blaney-Davidson E, van Caam A, Lodder K, Ramos Y, Kloppenburg M, Meulenbelt I, van der Kraan P, Goumans MJ, Ten Dijke P. (2021) Cripto favors chondrocyte hypertrophy via TGF-β SMAD1/5 signaling during development of osteoarthritis. J Pathol. 255 (3): 330-342.
[20]	Vig S, Buitinga M, Rondas D, Crèvecoeur I, van Zandvoort M, Waelkens E, Eizirik DL, Gysemans C, Baatsen P, Mathieu C, Overbergh L. (2019) Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells. Cell Death Dis. 10 (4): 309.
[21]	Strizzi L, Bianco C, Normanno N, Salomon D. (2005) Cripto-1: a multifunctional modulator during embryogenesis and oncogenesis. Oncogene. 24 (37): 5731-41.
[22]	McBratney-Owen B, Iseki S, Bamforth SD, Olsen BR, Morriss-Kay GM. (2008) Development and tissue origins of the mammalian cranial base. Dev Biol. 322 (1): 121-32.

Cite This Article

Plain Text BibTeX RIS

APA Style

Yuko Niidome-Matsuda, Junko Hatakeyama, Masaaki Takezaki, Masahiro Yoneda, Yuji Hatakeyama, et al. (2022). Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. American Journal of BioScience, 10(1), 31-34. https://doi.org/10.11648/j.ajbio.20221001.15

Copy | Download

ACS Style

Yuko Niidome-Matsuda; Junko Hatakeyama; Masaaki Takezaki; Masahiro Yoneda; Yuji Hatakeyama, et al. Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. Am. J. BioScience 2022, 10(1), 31-34. doi: 10.11648/j.ajbio.20221001.15

Copy | Download

AMA Style

Yuko Niidome-Matsuda, Junko Hatakeyama, Masaaki Takezaki, Masahiro Yoneda, Yuji Hatakeyama, et al. Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. Am J BioScience. 2022;10(1):31-34. doi: 10.11648/j.ajbio.20221001.15

Copy | Download

@article{10.11648/j.ajbio.20221001.15,
  author = {Yuko Niidome-Matsuda and Junko Hatakeyama and Masaaki Takezaki and Masahiro Yoneda and Yuji Hatakeyama and Sachio Tamaoki},
  title = {Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis},
  journal = {American Journal of BioScience},
  volume = {10},
  number = {1},
  pages = {31-34},
  doi = {10.11648/j.ajbio.20221001.15},
  url = {https://doi.org/10.11648/j.ajbio.20221001.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221001.15},
  abstract = {The synchondroses in the cranial base are important structures in craniofacial growth, and the spheno-occipital synchondrosis (SOS) is representative of a typical growth site. Endoplasmic reticulum (ER) stress is associated with multiple biological processes and is a critical factor in chondrogenesis. It has been reported that 78kDa Glucose-regulated protein (Grp78) plays an important role in suppressing regulators in ER stress-mediated apoptosis in chondrogenesis, and Cripto is the cell surface signaling partner of Grp78 in the transforming growth factor-β (TGF-β) signaling pathway. We attempt to clarify the immunolocalization of Grp78 and Cripto in the SOS. The mice head at embryonic day 17.5 (E17.5) were collected and embedded in paraffin. The serial sections were stained with hematoxylin and eosin, Alcian blue, lectin, and immunostaining. The SOS structure containing the resting, proliferative, and hypertrophic zone were identified with Alcian blue staining, wheat germ agglutinin, and Type II Collagen immunostaining. Immunostaining of Grp78 in the SOS revealed positive immunoreactivity in all the chondrocytes of the SOS. However, the chondrocytes of the proliferating zone were weakly immunopositive to Cripto, while the chondrocytes of the hypertrophic zone were strongly immunopositive. Since the immunolocalization of Grp78 and Cripto was different in cartilage zone, these data suggest that Grp78 and Cripto would be involved in the regulation of hypertrophic chondrocyte differentiation and may be related with ER stress in the SOS.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis
AU  - Yuko Niidome-Matsuda
AU  - Junko Hatakeyama
AU  - Masaaki Takezaki
AU  - Masahiro Yoneda
AU  - Yuji Hatakeyama
AU  - Sachio Tamaoki
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbio.20221001.15
DO  - 10.11648/j.ajbio.20221001.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 31
EP  - 34
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20221001.15
AB  - The synchondroses in the cranial base are important structures in craniofacial growth, and the spheno-occipital synchondrosis (SOS) is representative of a typical growth site. Endoplasmic reticulum (ER) stress is associated with multiple biological processes and is a critical factor in chondrogenesis. It has been reported that 78kDa Glucose-regulated protein (Grp78) plays an important role in suppressing regulators in ER stress-mediated apoptosis in chondrogenesis, and Cripto is the cell surface signaling partner of Grp78 in the transforming growth factor-β (TGF-β) signaling pathway. We attempt to clarify the immunolocalization of Grp78 and Cripto in the SOS. The mice head at embryonic day 17.5 (E17.5) were collected and embedded in paraffin. The serial sections were stained with hematoxylin and eosin, Alcian blue, lectin, and immunostaining. The SOS structure containing the resting, proliferative, and hypertrophic zone were identified with Alcian blue staining, wheat germ agglutinin, and Type II Collagen immunostaining. Immunostaining of Grp78 in the SOS revealed positive immunoreactivity in all the chondrocytes of the SOS. However, the chondrocytes of the proliferating zone were weakly immunopositive to Cripto, while the chondrocytes of the hypertrophic zone were strongly immunopositive. Since the immunolocalization of Grp78 and Cripto was different in cartilage zone, these data suggest that Grp78 and Cripto would be involved in the regulation of hypertrophic chondrocyte differentiation and may be related with ER stress in the SOS.
VL  - 10
IS  - 1
ER  -

Copy | Download

Author Information

Yuko Niidome-Matsuda

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Junko Hatakeyama

Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College, Fukuoka, Japan
Masaaki Takezaki

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Masahiro Yoneda

Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College, Fukuoka, Japan
Yuji Hatakeyama

Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
Sachio Tamaoki

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Yuko Niidome-Matsuda, Junko Hatakeyama, Masaaki Takezaki, Masahiro Yoneda, Yuji Hatakeyama, et al. (2022). Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. American Journal of BioScience, 10(1), 31-34. https://doi.org/10.11648/j.ajbio.20221001.15

Copy | Download

ACS Style

Yuko Niidome-Matsuda; Junko Hatakeyama; Masaaki Takezaki; Masahiro Yoneda; Yuji Hatakeyama, et al. Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. Am. J. BioScience 2022, 10(1), 31-34. doi: 10.11648/j.ajbio.20221001.15

Copy | Download

AMA Style

Yuko Niidome-Matsuda, Junko Hatakeyama, Masaaki Takezaki, Masahiro Yoneda, Yuji Hatakeyama, et al. Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis. Am J BioScience. 2022;10(1):31-34. doi: 10.11648/j.ajbio.20221001.15

Copy | Download

@article{10.11648/j.ajbio.20221001.15,
  author = {Yuko Niidome-Matsuda and Junko Hatakeyama and Masaaki Takezaki and Masahiro Yoneda and Yuji Hatakeyama and Sachio Tamaoki},
  title = {Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis},
  journal = {American Journal of BioScience},
  volume = {10},
  number = {1},
  pages = {31-34},
  doi = {10.11648/j.ajbio.20221001.15},
  url = {https://doi.org/10.11648/j.ajbio.20221001.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221001.15},
  abstract = {The synchondroses in the cranial base are important structures in craniofacial growth, and the spheno-occipital synchondrosis (SOS) is representative of a typical growth site. Endoplasmic reticulum (ER) stress is associated with multiple biological processes and is a critical factor in chondrogenesis. It has been reported that 78kDa Glucose-regulated protein (Grp78) plays an important role in suppressing regulators in ER stress-mediated apoptosis in chondrogenesis, and Cripto is the cell surface signaling partner of Grp78 in the transforming growth factor-β (TGF-β) signaling pathway. We attempt to clarify the immunolocalization of Grp78 and Cripto in the SOS. The mice head at embryonic day 17.5 (E17.5) were collected and embedded in paraffin. The serial sections were stained with hematoxylin and eosin, Alcian blue, lectin, and immunostaining. The SOS structure containing the resting, proliferative, and hypertrophic zone were identified with Alcian blue staining, wheat germ agglutinin, and Type II Collagen immunostaining. Immunostaining of Grp78 in the SOS revealed positive immunoreactivity in all the chondrocytes of the SOS. However, the chondrocytes of the proliferating zone were weakly immunopositive to Cripto, while the chondrocytes of the hypertrophic zone were strongly immunopositive. Since the immunolocalization of Grp78 and Cripto was different in cartilage zone, these data suggest that Grp78 and Cripto would be involved in the regulation of hypertrophic chondrocyte differentiation and may be related with ER stress in the SOS.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Immunohistochemical Study of 78kDa Glucose-regulated Protein (Grp78) and Cripto in the Spheno-occipital Synchondrosis
AU  - Yuko Niidome-Matsuda
AU  - Junko Hatakeyama
AU  - Masaaki Takezaki
AU  - Masahiro Yoneda
AU  - Yuji Hatakeyama
AU  - Sachio Tamaoki
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbio.20221001.15
DO  - 10.11648/j.ajbio.20221001.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 31
EP  - 34
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20221001.15
AB  - The synchondroses in the cranial base are important structures in craniofacial growth, and the spheno-occipital synchondrosis (SOS) is representative of a typical growth site. Endoplasmic reticulum (ER) stress is associated with multiple biological processes and is a critical factor in chondrogenesis. It has been reported that 78kDa Glucose-regulated protein (Grp78) plays an important role in suppressing regulators in ER stress-mediated apoptosis in chondrogenesis, and Cripto is the cell surface signaling partner of Grp78 in the transforming growth factor-β (TGF-β) signaling pathway. We attempt to clarify the immunolocalization of Grp78 and Cripto in the SOS. The mice head at embryonic day 17.5 (E17.5) were collected and embedded in paraffin. The serial sections were stained with hematoxylin and eosin, Alcian blue, lectin, and immunostaining. The SOS structure containing the resting, proliferative, and hypertrophic zone were identified with Alcian blue staining, wheat germ agglutinin, and Type II Collagen immunostaining. Immunostaining of Grp78 in the SOS revealed positive immunoreactivity in all the chondrocytes of the SOS. However, the chondrocytes of the proliferating zone were weakly immunopositive to Cripto, while the chondrocytes of the hypertrophic zone were strongly immunopositive. Since the immunolocalization of Grp78 and Cripto was different in cartilage zone, these data suggest that Grp78 and Cripto would be involved in the regulation of hypertrophic chondrocyte differentiation and may be related with ER stress in the SOS.
VL  - 10
IS  - 1
ER  -

Copy | Download