The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats

Eudoxie Pepelassi; Ioanna Xynogala; Despina Perrea; Alkistis Pantopoulou; George Agrogiannis; Ioannis Vrotsos

doi:doi:10.11648/j.ajim.20200803.12

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The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats

Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, Ioannis Vrotsos

Published in American Journal of Internal Medicine (Volume 8, Issue 3)

Received: 8 March 2020 Accepted: 3 April 2020 Published: 30 April 2020

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Abstract

The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.

Published in	American Journal of Internal Medicine (Volume 8, Issue 3)
DOI	10.11648/j.ajim.20200803.12
Page(s)	101-106
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Diabetes Mellitus, Type 1, Insulin, Periodontitis, Interleukin-18, Leptin, Biologic Markers

References

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APA Style

Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. (2020). The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. American Journal of Internal Medicine, 8(3), 101-106. https://doi.org/10.11648/j.ajim.20200803.12

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ACS Style

Eudoxie Pepelassi; Ioanna Xynogala; Despina Perrea; Alkistis Pantopoulou; George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am. J. Intern. Med. 2020, 8(3), 101-106. doi: 10.11648/j.ajim.20200803.12

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AMA Style

Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am J Intern Med. 2020;8(3):101-106. doi: 10.11648/j.ajim.20200803.12

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@article{10.11648/j.ajim.20200803.12,
  author = {Eudoxie Pepelassi and Ioanna Xynogala and Despina Perrea and Alkistis Pantopoulou and George Agrogiannis and Ioannis Vrotsos},
  title = {The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats},
  journal = {American Journal of Internal Medicine},
  volume = {8},
  number = {3},
  pages = {101-106},
  doi = {10.11648/j.ajim.20200803.12},
  url = {https://doi.org/10.11648/j.ajim.20200803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20200803.12},
  abstract = {The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.},
 year = {2020}
}

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TY  - JOUR
T1  - The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats
AU  - Eudoxie Pepelassi
AU  - Ioanna Xynogala
AU  - Despina Perrea
AU  - Alkistis Pantopoulou
AU  - George Agrogiannis
AU  - Ioannis Vrotsos
Y1  - 2020/04/30
PY  - 2020
N1  - https://doi.org/10.11648/j.ajim.20200803.12
DO  - 10.11648/j.ajim.20200803.12
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 101
EP  - 106
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20200803.12
AB  - The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.
VL  - 8
IS  - 3
ER  -

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Author Information

Eudoxie Pepelassi

Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
Ioanna Xynogala

Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
Despina Perrea

Laboratory for Experimental Surgery & Surgical Research “N. S. Christeas”, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece
Alkistis Pantopoulou

Laboratory for Experimental Surgery & Surgical Research “N. S. Christeas”, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece
George Agrogiannis

1^st Department of Pathology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece
Ioannis Vrotsos

Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece

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Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. (2020). The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. American Journal of Internal Medicine, 8(3), 101-106. https://doi.org/10.11648/j.ajim.20200803.12

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ACS Style

Eudoxie Pepelassi; Ioanna Xynogala; Despina Perrea; Alkistis Pantopoulou; George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am. J. Intern. Med. 2020, 8(3), 101-106. doi: 10.11648/j.ajim.20200803.12

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AMA Style

Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am J Intern Med. 2020;8(3):101-106. doi: 10.11648/j.ajim.20200803.12

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@article{10.11648/j.ajim.20200803.12,
  author = {Eudoxie Pepelassi and Ioanna Xynogala and Despina Perrea and Alkistis Pantopoulou and George Agrogiannis and Ioannis Vrotsos},
  title = {The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats},
  journal = {American Journal of Internal Medicine},
  volume = {8},
  number = {3},
  pages = {101-106},
  doi = {10.11648/j.ajim.20200803.12},
  url = {https://doi.org/10.11648/j.ajim.20200803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20200803.12},
  abstract = {The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.},
 year = {2020}
}

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TY  - JOUR
T1  - The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats
AU  - Eudoxie Pepelassi
AU  - Ioanna Xynogala
AU  - Despina Perrea
AU  - Alkistis Pantopoulou
AU  - George Agrogiannis
AU  - Ioannis Vrotsos
Y1  - 2020/04/30
PY  - 2020
N1  - https://doi.org/10.11648/j.ajim.20200803.12
DO  - 10.11648/j.ajim.20200803.12
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 101
EP  - 106
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20200803.12
AB  - The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.
VL  - 8
IS  - 3
ER  -

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