Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam

Cao Ngoc Diep; Pham Viet Cuong

doi:doi:10.11648/j.ajls.20130101.13

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Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam

Cao Ngoc Diep, Pham Viet Cuong

Published in American Journal of Life Sciences (Volume 1, Issue 1)

Published: 20 February 2013

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Abstract

A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.

Published in	American Journal of Life Sciences (Volume 1, Issue 1)
DOI	10.11648/j.ajls.20130101.13
Page(s)	14-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Heterotrophic Nitrogen Removal, 16S RNA Gene Sequence, Biologic Nitrogen Removal, Piggery Waste, Gram-Positive Bacteria

References

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[3]	J.J. Su, R.S. Yeh, and P.W. Tseng, "A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan", Curr. Microb. vol. 53, pp. 77-81, 2006.
[4]	L.A. Robertson, E.W.J. Van Neil, R.A.M. Torremans, and J.G. Kuenen, "Simultaneous nitrification and denitrification in aerobic chemost at cultures of Thiosphaera pantotropha" Appl. Environ. Microb. vol. 54, pp. 2812-2818, 1988.
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[7]	B. Zhao, Y.L. He, J. Hughes, and X.F. Zhang, "Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoa-ceticus HNR", Biores. Technol., vol.101, pp. 5194-5200, 2010.
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APA Style

Cao Ngoc Diep, Pham Viet Cuong. (2013). Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. American Journal of Life Sciences, 1(1), 14-21. https://doi.org/10.11648/j.ajls.20130101.13

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Cao Ngoc Diep; Pham Viet Cuong. Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. Am. J. Life Sci. 2013, 1(1), 14-21. doi: 10.11648/j.ajls.20130101.13

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AMA Style

Cao Ngoc Diep, Pham Viet Cuong. Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. Am J Life Sci. 2013;1(1):14-21. doi: 10.11648/j.ajls.20130101.13

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@article{10.11648/j.ajls.20130101.13,
  author = {Cao Ngoc Diep and Pham Viet Cuong},
  title = {Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {1},
  pages = {14-21},
  doi = {10.11648/j.ajls.20130101.13},
  url = {https://doi.org/10.11648/j.ajls.20130101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130101.13},
  abstract = {A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.},
 year = {2013}
}

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TY  - JOUR
T1  - Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam
AU  - Cao Ngoc Diep
AU  - Pham Viet Cuong
Y1  - 2013/02/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130101.13
DO  - 10.11648/j.ajls.20130101.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 14
EP  - 21
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130101.13
AB  - A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.
VL  - 1
IS  - 1
ER  -

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Author Information

Cao Ngoc Diep

Dept. Microbiology Biotechnology, Can Tho City, Vietnam
Pham Viet Cuong

Dept. Microbiology Biotechnology, Can Tho City, Vietnam

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APA Style

Cao Ngoc Diep, Pham Viet Cuong. (2013). Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. American Journal of Life Sciences, 1(1), 14-21. https://doi.org/10.11648/j.ajls.20130101.13

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ACS Style

Cao Ngoc Diep; Pham Viet Cuong. Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. Am. J. Life Sci. 2013, 1(1), 14-21. doi: 10.11648/j.ajls.20130101.13

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AMA Style

Cao Ngoc Diep, Pham Viet Cuong. Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam. Am J Life Sci. 2013;1(1):14-21. doi: 10.11648/j.ajls.20130101.13

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@article{10.11648/j.ajls.20130101.13,
  author = {Cao Ngoc Diep and Pham Viet Cuong},
  title = {Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {1},
  pages = {14-21},
  doi = {10.11648/j.ajls.20130101.13},
  url = {https://doi.org/10.11648/j.ajls.20130101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130101.13},
  abstract = {A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.},
 year = {2013}
}

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TY  - JOUR
T1  - Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam
AU  - Cao Ngoc Diep
AU  - Pham Viet Cuong
Y1  - 2013/02/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130101.13
DO  - 10.11648/j.ajls.20130101.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 14
EP  - 21
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130101.13
AB  - A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.
VL  - 1
IS  - 1
ER  -

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