Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam

Cao Ngoc Diep; Than Ngoc Hieu

doi:doi:10.11648/j.ajls.20130103.12

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Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam

Cao Ngoc Diep, Than Ngoc Hieu

Published in American Journal of Life Sciences (Volume 1, Issue 3)

Received: 2 May 2013 Published: 10 June 2013

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Abstract

Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.

Published in	American Journal of Life Sciences (Volume 1, Issue 3)
DOI	10.11648/j.ajls.20130103.12
Page(s)	88-92
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Bacillus Coagulans, Bacillus Megaterium, Denatured Rock, Identification, Phosphate and Potassium Solubilization

References

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[2]	C.S.Cockell, D. Pybus, K. Olsson-Francis, L. Kelly, D. Petley, N. Rosser, K. Howard, and F. Mosselmans, Molecular Characterization and Geological Microenvironment of a Microbial Community Inhabiting Weathering Receding Shale Cliffs, Microb. Ecol. vol (61), 2011, pp. 166-181.
[3]	H. Xiufang, C. Jishuang and G. Jiangfeng, Two phosphate- and potassium- solubilizing bacteria isolated from Tianmu Moutain, Zhejiang, China. World Journal of Microbiology and Biotechnology. vol (22), 2006, pp. 983 - 990.
[4]	N.V. Thanh, V.T.Xuan, L.Q.Tri and V.Q.Minh, Studies on main crop systems cultivated on sulfaquept soils in Mekong Delta. MSc. Thesis, Can Tho University, Vietnam. 1997
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[6]	A.H. Goldstein, Bacterial solubilization of mineral phosphates: historical perspective and furture prospects. American Journal of Alternative Agriculture1, 1986, pp.51- 57.
[7]	V.G. Aleksandrov, R.N. Blagodyr and I.P. Ilev, Liberation of phosphoric aicd from apatite by silicate bacteria. Mikrobiolohichnyi Zhurnal (Kiev) vol(29), 1967, pp.111 - 114.
[8]	M. Manib, M.K. Zahra, A.L. Abdel, and A. Heggo, Role of solicate bacteria in releasing K and Si from biotite and orthoclase. In. Soil biology and Conservation of the Biosphere, ed. Szegi J. 1986, pp.733-743. Budapest, Akademiai Kiado, ISBN 9630537001.
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[11]	M. Datta, S. Banish, and R.K. Dupta, Studies on the efficiency of a phytohormone producing phosphate solubilizing Bacillus firmus in augmenting paddy yield in acid soils of Nagaland. Plant and Soil vol (69), 1982, pp.365-373.
[12]	C. N. Diep, T. T. Phong and T. T. Giang, Isolation and Selection of phosphate-solubilizing and IAA-biosynthesis Pseudomonas sp. Science and technology J. Agriculture and Rural Development. Ministry of Agriculture and Rural Development, Vietnam vol (9), 2009, pp.32-35.
[13]	Z.A. Burgstaller, H. Sreasser, H. Wobking and F. Shinner, Solubilization of zinc oxide from filterdust with Penicillium simplicassimum bioreactor, leaching and stoichiometry. Environmental Science and Technology vol (26), 1992, pp.340-346.
[14]	G.G. Nianikova, E.E. Kuprina, O.V. Pestova, and S.V. Vodolazhskaia, Immobilizing of Bacillus mucilaginosus, a producer of exopolysaccharides, on chitin. Priklalmaia Biokhilia I Mikrobiologiya vol (38), 2002, pp. 300-304.
[15]	E. Bird, Coastal geomorphology: an introduction, John Wiley and Sons, Incs, New York, 2000.
[16]	S.A. Welch, W.W. Barker, and J.F. Banfield, Microbial extracellular polysaccharides and plagioclase dissolution. Geochim Cosmochim. Acta. vol (63), 1999, pp.1405-1419.
[17]	P.C. Bennett, J.R. Rogers, W.J. Choi, and F.K. Hiebert, Silicates, silicate weathering, and microbial ecology. Geomicrobiol. J. vol (18), 2001, pp.3-19.
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APA Style

Cao Ngoc Diep, Than Ngoc Hieu. (2013). Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. American Journal of Life Sciences, 1(3), 88-92. https://doi.org/10.11648/j.ajls.20130103.12

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ACS Style

Cao Ngoc Diep; Than Ngoc Hieu. Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. Am. J. Life Sci. 2013, 1(3), 88-92. doi: 10.11648/j.ajls.20130103.12

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AMA Style

Cao Ngoc Diep, Than Ngoc Hieu. Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. Am J Life Sci. 2013;1(3):88-92. doi: 10.11648/j.ajls.20130103.12

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@article{10.11648/j.ajls.20130103.12,
  author = {Cao Ngoc Diep and Than Ngoc Hieu},
  title = {Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {3},
  pages = {88-92},
  doi = {10.11648/j.ajls.20130103.12},
  url = {https://doi.org/10.11648/j.ajls.20130103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130103.12},
  abstract = {Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.},
 year = {2013}
}

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TY  - JOUR
T1  - Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam
AU  - Cao Ngoc Diep
AU  - Than Ngoc Hieu
Y1  - 2013/06/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130103.12
DO  - 10.11648/j.ajls.20130103.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 88
EP  - 92
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130103.12
AB  - Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.
VL  - 1
IS  - 3
ER  -

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Author Information

Cao Ngoc Diep

Dept. Microbiology Biotechnology, Depertment of Rural Development, Can Tho City, Vietnam
Than Ngoc Hieu

Dept. Microbiology Biotechnology, Depertment of Rural Development, Can Tho City, Vietnam

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APA Style

Cao Ngoc Diep, Than Ngoc Hieu. (2013). Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. American Journal of Life Sciences, 1(3), 88-92. https://doi.org/10.11648/j.ajls.20130103.12

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ACS Style

Cao Ngoc Diep; Than Ngoc Hieu. Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. Am. J. Life Sci. 2013, 1(3), 88-92. doi: 10.11648/j.ajls.20130103.12

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AMA Style

Cao Ngoc Diep, Than Ngoc Hieu. Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam. Am J Life Sci. 2013;1(3):88-92. doi: 10.11648/j.ajls.20130103.12

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@article{10.11648/j.ajls.20130103.12,
  author = {Cao Ngoc Diep and Than Ngoc Hieu},
  title = {Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {3},
  pages = {88-92},
  doi = {10.11648/j.ajls.20130103.12},
  url = {https://doi.org/10.11648/j.ajls.20130103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130103.12},
  abstract = {Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.},
 year = {2013}
}

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TY  - JOUR
T1  - Phosphate and Potassium Solubilizing Bacteria from Weathered Materials of denatured Rock Mountain, Ha Tien, Kien Giang Province, Vietnam
AU  - Cao Ngoc Diep
AU  - Than Ngoc Hieu
Y1  - 2013/06/10
PY  - 2013
N1  - https://doi.org/10.11648/j.ajls.20130103.12
DO  - 10.11648/j.ajls.20130103.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 88
EP  - 92
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20130103.12
AB  - Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l-1 P2O5 and >50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.
VL  - 1
IS  - 3
ER  -

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