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In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria

Received: 16 May 2013     Published: 20 June 2013
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Abstract

The inhibitory activity of seed extracts of Achyranthes aspera, a widely used folk medicinal plant in Bangladesh, was examined using methanol, acetone, ethyl acetate and petroleum sprit as solvents against five test bacteria by disc diffusion method. Methanol extract was found to reveal significant inhibitory activity against the pathogenic B. subtilis, E. coli and K. pneumoniae. Only the methanol and ethyl acetate extracts were effective against all bacteria and the best activity was found against B. subtilis in terms of zone of clearance. The best minimum inhibitory concentration value was found by methanol extract against B. subtilis. The present study suggests that the methanol extract of seed of this plant could be a possible source of obtaining new and effective herbal medicines to treat infections; hence, it justified the ethnic use.

Published in American Journal of Life Sciences (Volume 1, Issue 3)
DOI 10.11648/j.ajls.20130103.16
Page(s) 113-116
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Achyranthes Aspera, Inhibitory Activity, Medicinal Plant

References
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[3] Lakshmi NPV, Kishore KK, Mohan KC, Gunesh G and Narasimha RM. Antimicrobial activity of Achyranthes aspera. Biosci. Biotechnol. Res. Asia. 2006; 3: 1-2.
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[5] Valsaraj R, Pushpangadan P, Smitt UW, Andersen A and Nyman U. Antimicrobial screening of selected medicinal plants from India. J. Ethnopharmacol. 1997; 58: 75-83.
[6] Lai PK and Roy J. Antimicrobial and chemopreventive properties of herbs and spices. Cur. Med. Chem. 2004; 11: 1451-1460.
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[8] Anonymous. The Wealth of India, Raw Materials. New Delhi: Publication and Information Directorate, Center for Scientific and Industrial Research (CSIR). 1985; p57.
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[10] Singleton P. Bacteria in Biology, Biotechnology and Medicine. 4th edition. New York, John Wiley and Sons Ltd. 1999.
[11] Londonkar R, Reddy CV and Kumar AK. Potential antibacterial and antifungal activity of Achyranthes aspera L. Recent Res. Sci. Technol. 2011; 3(4): 53-57.
[12] Narayana RK, Reddy SM, Chaluvadi MR and Krishna DR. Bioflavanoids: classification, pharmacological, biochemical effects and therapeutic potentials. Indian J. Pharmacol. 2001; 33: 2-16.
[13] Gokhale AB, Damre AS, Kulkami KR and Saraf MN. Preliminary evaluation of anti-inflammatory and anti-arthritic activity of S. lappa, A. speciosa and A. aspera. J. Phytomed. 2002; 9(5): 433-437.
[14] Seher G, Dilek Turgut-Balik and Nazmi G. Antimicrobial activities and some fatty acids of Turmeric, Ginger root and Linseed used in treatment of infectious diseases. World J. Agri. Sci. 2006; 2(4): 439-442.
[15] Bauer AW, Kirby WM., Sherris JC and Turck M. Antibiotic susceptibility testing by a standardized single disc method. American J. Clin. Pathol. 1966; 45: 493-496.
[16] NCCLS. Performance standards for antimicrobial disk susceptibility tests: Approval standard M2-A7 7th edition. Pennsylvania: Clinical and Laboratory Standards Institute. 2000.
[17] Reiner R. Antibiotica: An Introduction. F. Hoffman (Ed.). La Roche and Co. Ltd. Switzerland. 1982; p23-30.
[18] Ashokkumar P, Rajkumar and Kanimozhi. Phytochemical screening and antimicrobial activity from five Indian medicinal plants against human pathogens. Middle-East J. Sci. Res. 2010; 5(6): 477-482.
[19] Varuna KM, Khan MU and Sharma PK. Review on Achyranthes aspera. J. Pharm. Res. 2010; 3(4): 714-717.
[20] Beaulah AG, Sadiq MA and Santhi JR. Antioxidant and antibacterial activity of Achyranthes aspera: An in vitro study. Der Pharma Chemica. 2011; 3(5): 255-262.
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  • APA Style

    Dash B. K., Sen M. K., Alam M. K. (2013). In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria. American Journal of Life Sciences, 1(3), 113-116. https://doi.org/10.11648/j.ajls.20130103.16

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    ACS Style

    Dash B. K.; Sen M. K.; Alam M. K. In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria. Am. J. Life Sci. 2013, 1(3), 113-116. doi: 10.11648/j.ajls.20130103.16

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    AMA Style

    Dash B. K., Sen M. K., Alam M. K. In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria. Am J Life Sci. 2013;1(3):113-116. doi: 10.11648/j.ajls.20130103.16

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  • @article{10.11648/j.ajls.20130103.16,
      author = {Dash B. K. and Sen M. K. and Alam M. K.},
      title = {In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria},
      journal = {American Journal of Life Sciences},
      volume = {1},
      number = {3},
      pages = {113-116},
      doi = {10.11648/j.ajls.20130103.16},
      url = {https://doi.org/10.11648/j.ajls.20130103.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130103.16},
      abstract = {The inhibitory activity of seed extracts of Achyranthes aspera, a widely used folk medicinal plant in Bangladesh, was examined using methanol, acetone, ethyl acetate and petroleum sprit as solvents against five test bacteria by disc diffusion method. Methanol extract was found to reveal significant inhibitory activity against the pathogenic B. subtilis, E. coli and K. pneumoniae. Only the methanol and ethyl acetate extracts were effective against all bacteria and the best activity was found against B. subtilis in terms of zone of clearance. The best minimum inhibitory concentration value was found by methanol extract against B. subtilis. The present study suggests that the methanol extract of seed of this plant could be a possible source of obtaining new and effective herbal medicines to treat infections; hence, it justified the ethnic use.},
     year = {2013}
    }
    

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    T1  - In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria
    AU  - Dash B. K.
    AU  - Sen M. K.
    AU  - Alam M. K.
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    DO  - 10.11648/j.ajls.20130103.16
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 113
    EP  - 116
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20130103.16
    AB  - The inhibitory activity of seed extracts of Achyranthes aspera, a widely used folk medicinal plant in Bangladesh, was examined using methanol, acetone, ethyl acetate and petroleum sprit as solvents against five test bacteria by disc diffusion method. Methanol extract was found to reveal significant inhibitory activity against the pathogenic B. subtilis, E. coli and K. pneumoniae. Only the methanol and ethyl acetate extracts were effective against all bacteria and the best activity was found against B. subtilis in terms of zone of clearance. The best minimum inhibitory concentration value was found by methanol extract against B. subtilis. The present study suggests that the methanol extract of seed of this plant could be a possible source of obtaining new and effective herbal medicines to treat infections; hence, it justified the ethnic use.
    VL  - 1
    IS  - 3
    ER  - 

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Author Information
  • Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh

  • Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh

  • Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh

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