Antidiabetic, Toxicity Studies and Phytochemical Profile of <i>Hedranthera barteri</i> (Hook. f.) Pichon

Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu; Akintunde Samuel Odediran; Farouk Adedeji Oladoja; Katherine Olabanjo Olufolabo; Adediwura Fred-Jaiyesimi

doi:doi:10.11648/j.jdmp.20230902.13

Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, Akintunde Samuel Odediran, Farouk Adedeji Oladoja, Katherine Olabanjo Olufolabo, Adediwura Fred-Jaiyesimi

Published in Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)

Received: 23 April 2023 Accepted: 17 May 2023 Published: 29 June 2023

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Abstract

Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and p< 0.05 was taken as significant. Results: The median lethal dose, LD₅₀ of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.

Published in	Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)
DOI	10.11648/j.jdmp.20230902.13
Page(s)	49-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Hedranthera barteri, Diabetes Mellitus, Toxicity, Phytochemical Profile

References

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Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. (2023). Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. Journal of Diseases and Medicinal Plants, 9(2), 49-59. https://doi.org/10.11648/j.jdmp.20230902.13

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Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J. Dis. Med. Plants 2023, 9(2), 49-59. doi: 10.11648/j.jdmp.20230902.13

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Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J Dis Med Plants. 2023;9(2):49-59. doi: 10.11648/j.jdmp.20230902.13

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@article{10.11648/j.jdmp.20230902.13,
  author = {Marcus Durojaye Ayoola and Kingsley Onyekachi Ngwu and Oluwaseun Alimot Kareem and Khadijah Adewunmi and Oluwabusayo Emmanuel Kasumu and Akintunde Samuel Odediran and Farouk Adedeji Oladoja and Katherine Olabanjo Olufolabo and Adediwura Fred-Jaiyesimi},
  title = {Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {49-59},
  doi = {10.11648/j.jdmp.20230902.13},
  url = {https://doi.org/10.11648/j.jdmp.20230902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.13},
  abstract = {Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.},
 year = {2023}
}

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TY  - JOUR
T1  - Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon
AU  - Marcus Durojaye Ayoola
AU  - Kingsley Onyekachi Ngwu
AU  - Oluwaseun Alimot Kareem
AU  - Khadijah Adewunmi
AU  - Oluwabusayo Emmanuel Kasumu
AU  - Akintunde Samuel Odediran
AU  - Farouk Adedeji Oladoja
AU  - Katherine Olabanjo Olufolabo
AU  - Adediwura Fred-Jaiyesimi
Y1  - 2023/06/29
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.13
DO  - 10.11648/j.jdmp.20230902.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 49
EP  - 59
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.13
AB  - Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.
VL  - 9
IS  - 2
ER  -

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Marcus Durojaye Ayoola

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Kingsley Onyekachi Ngwu

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Oluwaseun Alimot Kareem

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Khadijah Adewunmi

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Oluwabusayo Emmanuel Kasumu

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Akintunde Samuel Odediran

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Farouk Adedeji Oladoja

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Katherine Olabanjo Olufolabo

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Adediwura Fred-Jaiyesimi

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria

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Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. (2023). Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. Journal of Diseases and Medicinal Plants, 9(2), 49-59. https://doi.org/10.11648/j.jdmp.20230902.13

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Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J. Dis. Med. Plants 2023, 9(2), 49-59. doi: 10.11648/j.jdmp.20230902.13

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AMA Style

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J Dis Med Plants. 2023;9(2):49-59. doi: 10.11648/j.jdmp.20230902.13

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@article{10.11648/j.jdmp.20230902.13,
  author = {Marcus Durojaye Ayoola and Kingsley Onyekachi Ngwu and Oluwaseun Alimot Kareem and Khadijah Adewunmi and Oluwabusayo Emmanuel Kasumu and Akintunde Samuel Odediran and Farouk Adedeji Oladoja and Katherine Olabanjo Olufolabo and Adediwura Fred-Jaiyesimi},
  title = {Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {49-59},
  doi = {10.11648/j.jdmp.20230902.13},
  url = {https://doi.org/10.11648/j.jdmp.20230902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.13},
  abstract = {Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.},
 year = {2023}
}

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TY  - JOUR
T1  - Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon
AU  - Marcus Durojaye Ayoola
AU  - Kingsley Onyekachi Ngwu
AU  - Oluwaseun Alimot Kareem
AU  - Khadijah Adewunmi
AU  - Oluwabusayo Emmanuel Kasumu
AU  - Akintunde Samuel Odediran
AU  - Farouk Adedeji Oladoja
AU  - Katherine Olabanjo Olufolabo
AU  - Adediwura Fred-Jaiyesimi
Y1  - 2023/06/29
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.13
DO  - 10.11648/j.jdmp.20230902.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 49
EP  - 59
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.13
AB  - Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.
VL  - 9
IS  - 2
ER  -

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