The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage

Sun Yu-hao; Cui Shi-gang; Zhang Yong-li

doi:doi:10.11648/j.mc.20221002.11

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The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage

Sun Yu-hao, Cui Shi-gang, Zhang Yong-li

Published in Modern Chemistry (Volume 10, Issue 2)

Received: 18 April 2022 Published: 20 April 2022

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Abstract

In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240μmol·m^-2·s^-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.

Published in	Modern Chemistry (Volume 10, Issue 2)
DOI	10.11648/j.mc.20221002.11
Page(s)	23-29
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Haematococcus Pluvialis, Astaxanthin, pH

References

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[2]	Wang Xiaodan, Liu Baoling, Li Runzhi. Responses of metabolites and mineral elements in Haematococcus pluvialis to high light [J]. journal of shanxi agricultural sciences, 2021, 49 (11): 1280-1285.
[3]	Li Wenxue, Bai Huiying, Zhao Hongjing. Study on Optimization of Astaxanthin Accumulation Conditions of Haematococcus pluvialis [J]. Food Research and Development, 2021, 42 (16): 152-156.
[4]	Wu Qilu, Cui Wenqian, Shen Liang. Effects of environmental factors on main components of extracellular polymeric substances in microalgae [J]. Journal of Xiamen University, 2018, 57 (03): 346-353.
[5]	Hu Hongwei, Yan Lingpeng, Ma Xiaotao. Research Progress on Application of Astaxanthin in Aquaculture [J]. Feed Panorama, 2021 (07): 10-18.
[6]	Li Yan, Gao Jing. Research progress on extraction of natural astaxanthin from Haematococcus pluvialis [J]. food and machinery, 2020, 36 (12): 211-217.
[7]	Yue Chen, Yu Xuya, Zhao Yongteng. Effects of exogenous 2, 6-di-tert-butyl-p-cresol on astaxanthin accumulation in Haematococcus pluvialis under abiotic stress conditions [J]. Microbiology, 2019, 46 (06): 1265-1271.
[8]	Li Jiayi, Dou Yong, Shao Peng. Optimization of Culture Conditions for Astaxanthin Production by Haematococcus pluvialis by Response Surface Methodology [J]. Journal of Tianjin Agricultural College, 2019, 26 (03): 57-61.
[9]	Wang Shuyan, Meng Yingying, Xue Song. Review of Astaxanthin Detection Methods in Haematococcus pluvialis [J]. Journal of Inner Mongolia University for Nationalities, 2020, 35 (02): 102-107.
[10]	Zhu Qin, Zhang Chunhui, Shi Ying. Effects of light quality on astaxanthin synthesis and lipid accumulation in Haematococcus pluvialis [J]. Acta Phytophysiology Sinica, 2021, 57 (09): 1778-1788.
[11]	Li Qingqing. Mechanism of salt stress coupled with GABA on growth and astaxanthin biosynthesis in Haematococcus pluvialis [D]. kunming university of science and technology, 2021.
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[13]	Gao Qiuying, Wang Lili, Wang Rongzhong. Study on Least Square Curve Fitting and Optimization Algorithm [J]. process computer, 2021, 34 (11): 100-101.

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APA Style

Sun Yu-hao, Cui Shi-gang, Zhang Yong-li. (2022). The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Modern Chemistry, 10(2), 23-29. https://doi.org/10.11648/j.mc.20221002.11

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ACS Style

Sun Yu-hao; Cui Shi-gang; Zhang Yong-li. The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Mod. Chem. 2022, 10(2), 23-29. doi: 10.11648/j.mc.20221002.11

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AMA Style

Sun Yu-hao, Cui Shi-gang, Zhang Yong-li. The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Mod Chem. 2022;10(2):23-29. doi: 10.11648/j.mc.20221002.11

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@article{10.11648/j.mc.20221002.11,
  author = {Sun Yu-hao and Cui Shi-gang and Zhang Yong-li},
  title = {The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage},
  journal = {Modern Chemistry},
  volume = {10},
  number = {2},
  pages = {23-29},
  doi = {10.11648/j.mc.20221002.11},
  url = {https://doi.org/10.11648/j.mc.20221002.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.mc.20221002.11},
  abstract = {In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240μmol·m-2·s-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.},
 year = {2022}
}

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TY  - JOUR
T1  - The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage
AU  - Sun Yu-hao
AU  - Cui Shi-gang
AU  - Zhang Yong-li
Y1  - 2022/04/20
PY  - 2022
N1  - https://doi.org/10.11648/j.mc.20221002.11
DO  - 10.11648/j.mc.20221002.11
T2  - Modern Chemistry
JF  - Modern Chemistry
JO  - Modern Chemistry
SP  - 23
EP  - 29
PB  - Science Publishing Group
SN  - 2329-180X
UR  - https://doi.org/10.11648/j.mc.20221002.11
AB  - In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240μmol·m-2·s-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.
VL  - 10
IS  - 2
ER  -

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Author Information

Sun Yu-hao

School of Automation and Electrical Engineering, Tianjin University of Technology and Education, Tianjin, China
Cui Shi-gang

School of Automation and Electrical Engineering, Tianjin University of Technology and Education, Tianjin, China
Zhang Yong-li

School of Automation and Electrical Engineering, Tianjin University of Technology and Education, Tianjin, China

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Plain Text BibTeX RIS

APA Style

Sun Yu-hao, Cui Shi-gang, Zhang Yong-li. (2022). The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Modern Chemistry, 10(2), 23-29. https://doi.org/10.11648/j.mc.20221002.11

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ACS Style

Sun Yu-hao; Cui Shi-gang; Zhang Yong-li. The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Mod. Chem. 2022, 10(2), 23-29. doi: 10.11648/j.mc.20221002.11

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AMA Style

Sun Yu-hao, Cui Shi-gang, Zhang Yong-li. The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage. Mod Chem. 2022;10(2):23-29. doi: 10.11648/j.mc.20221002.11

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@article{10.11648/j.mc.20221002.11,
  author = {Sun Yu-hao and Cui Shi-gang and Zhang Yong-li},
  title = {The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage},
  journal = {Modern Chemistry},
  volume = {10},
  number = {2},
  pages = {23-29},
  doi = {10.11648/j.mc.20221002.11},
  url = {https://doi.org/10.11648/j.mc.20221002.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.mc.20221002.11},
  abstract = {In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240μmol·m-2·s-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage
AU  - Sun Yu-hao
AU  - Cui Shi-gang
AU  - Zhang Yong-li
Y1  - 2022/04/20
PY  - 2022
N1  - https://doi.org/10.11648/j.mc.20221002.11
DO  - 10.11648/j.mc.20221002.11
T2  - Modern Chemistry
JF  - Modern Chemistry
JO  - Modern Chemistry
SP  - 23
EP  - 29
PB  - Science Publishing Group
SN  - 2329-180X
UR  - https://doi.org/10.11648/j.mc.20221002.11
AB  - In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240μmol·m-2·s-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.
VL  - 10
IS  - 2
ER  -

Copy | Download