Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of <i>Merremia tridentata</i> Leaves

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall

doi:doi:10.11648/j.sjc.20221004.14

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Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall

Published in Science Journal of Chemistry (Volume 10, Issue 4)

Received: 3 August 2022 Accepted: 18 August 2022 Published: 29 August 2022

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Abstract

The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.

Published in	Science Journal of Chemistry (Volume 10, Issue 4)
DOI	10.11648/j.sjc.20221004.14
Page(s)	120-125
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Merremia tridentata, Extraction, Antioxidant, Polyphenols, Flavonoids, Proanthocyanidins

References

[1]	Gaye, A. A., Ibn, O., Cisse, K., Ndiaye, B., Ayessou, N. C., Cisse, M., & Diop, C. M. (2019). Evaluation of Phenolic Content and Antioxidant Activity of Aqueous Extracts of Three Carica papaya Varieties Cultivated in Senegal. Food and Nutrition Sciences, 10 (3), 276–289. https://doi.org/10.4236/fns.2019.103021
[2]	Pincemail, J., Bonjean, K., Cayeux, K., & Defraigne, J.-O. (2002). Mécanismes physiologiques de la défense antioxydante. Nutrition Clinique et Métabolisme, 16 (4), 233–239. https://doi.org/10.1016/S0985-0562(02)00166-8
[3]	Pandey, V. P., Singh, S., Jaiswal, N., Awasthi, M., Pandey, B., & Dwivedi, U. N. (2013). Papaya fruit ripening: ROS metabolism, gene cloning, characterization and molecular docking of peroxidase. Journal of Molecular Catalysis B: Enzymatic, 98, 98–105. https://doi.org/10.1016/j.molcatb.2013.10.005
[4]	Abdel-Hameed, E.-S. S. (2009). Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chemistry, 114 (4), 1271–1277. https://doi.org/10.1016/j.foodchem.2008.11.005
[5]	Pande, D., Negi, R., Khanna, R. S., & Khanna, H. D. (2011). Protein Damage and Antioxidant Status Alterations Caused by Oxidative Injury in Chronic Myeloid Leukemia. The Einstein Journal of Biology and Medicine, 27, 55–58. https://www.einsteinmed.edu/uploadedFiles/Pulications/EJBM/27.2%20Pande%207-10.pdf
[6]	Adebiyi, O. A., Ameh, D. A., Onyike, E., & James, D. B. (2021). Acute and Chronic Toxicity Studies of Ethanol Leaf Extract of Merremia Tridentata (Linn) Hallier F. European Journal of Advanced Chemistry Research, 2 (3), 14–20. https://doi.org/10.24018/ejchem.2021.2.3.62
[7]	Adebiyi, O. A., Ameh, D. A., Onyike, E., & James, D. B. (2022). Anti-Hyperglycaemic and Anti-Oxidative Effect of Merremia Tridentata (Linn) Hallier F Ethanol Leaf Extract. European Journal of Advanced Chemistry Research, 3 (1), 47–51. https://doi.org/10.24018/ejchem.2022.3.1.87
[8]	Sharma, P., & Gupta, G. (2022). Phytochemical, pharmacological, and pharmacognostic overview of Merremia tridentata L. Hallier. Journal of Applied Biology and Biotechnology, 10 (3), 219–224. 10.7324/JABB.2022.100325
[9]	Vuong, Q. V, Hirun, S., Roach, P. D., Bowyer, M. C., Phillips, P. A., & Scarlett, C. J. (2013). Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. Journal of Herbal Medicine, 3 (3), 104–111. https://doi.org/10.1016/j.hermed.2013.04.004
[10]	Mohdaly, A. A. A., Smetanska, I., Ramadan, M. F., Sarhan, M. A., & Mahmoud, A. (2011). Antioxidant potential of sesame (Sesamum indicum) cake extract in stabilization of sunflower and soybean oils. Industrial Crops and Products, 34 (1), 952–959. https://doi.org/10.1016/j.indcrop.2011.02.018
[11]	Gurnani, N., Gupta, M., Mehta, D., & Mehta, B. K. (2016). Chemical composition, total phenolic and flavonoid contents, and in vitro antimicrobial and antioxidant activities of crude extracts from red chilli seeds (Capsicum frutescens L.). Journal of Taibah University for Science, 10 (4), 462–470. https://doi.org/10.1016/j.jtusci.2015.06.011
[12]	Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96 (2), 254–260. https://doi.org/10.1016/j.foodchem.2005.02.033
[13]	Meda, N. R., Fraisse, D., Gnoula, C., Vivier, M., Felgines, C., & Senejoux, F. (2017). Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assay. European Food Research and Technology, 243 (9), 1659–1666. https://doi.org/10.1007/s00217-017-2873-7
[14]	Rubio, C. P., Hernández-Ruiz, J., Martinez-Subiela, S., Tvarijonaviciute, A., & Ceron, J. J. (2016). Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update. BMC veterinary research, 12 (1), 166–166. https://doi.org/10.1186/s12917-016-0792-7
[15]	Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine: CUPRAC Method. Journal of Agricultural and Food Chemistry, 52 (26), 7970–7981. https://doi.org/10.1021/jf048741x
[16]	Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., & Núñez, M. J. (2005). Effect of Solvent, Temperature, and Solvent-to-Solid Ratio on the Total Phenolic Content and Antiradical Activity of Extracts from Different Components of Grape Pomace. Journal of Agricultural and Food Chemistry, 53 (6), 2111–2117. https://doi.org/10.1021/jf0488110
[17]	Ballard, T. S., Mallikarjunan, P., Zhou, K., & O’Keefe, S. F. (2009). Optimizing the extraction of phenolic antioxidants from peanut skins using response surface methodology. Journal of Agricultural and Food Chemistry, 57 (8), 3064–3072. https://doi.org/10.1021/jf8030925
[18]	Alu’datt, M. H., Alli, I., Ereifej, K., Alhamad, M. N., Alsaad, A., & Rababeh, T. (2011). Optimisation and characterisation of various extraction conditions of phenolic compounds and antioxidant activity in olive seeds. Natural Product Research, 25 (9), 876–889. https://doi.org/10.1080/14786419.2010.489048
[19]	Gertenbach, D. (2002). Solid-Liquid Extraction Technologies for Manufacturing Nutraceuticals. In: J. Shi, G. Mazza, and M. L. Maguer, Eds., Functional Foods: Biochemical and Processing Aspects, CRC Press, USA, 2002, pp. 332-365. https://doi.org/10.1201/9781420012873
[20]	Ionita, P. (2021). The Chemistry of DPPH· Free Radical and Congeners. International Journal of Molecular Sciences, 22 (4), 1545. https://doi.org/10.3390/ijms22041545
[21]	Apak, R., Güçlü, K., Özyürek, M., & Çelik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160 (4), 413–419. https://doi.org/10.1007/s00604-007-0777-0

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APA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. (2022). Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Science Journal of Chemistry, 10(4), 120-125. https://doi.org/10.11648/j.sjc.20221004.14

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ACS Style

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci. J. Chem. 2022, 10(4), 120-125. doi: 10.11648/j.sjc.20221004.14

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AMA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci J Chem. 2022;10(4):120-125. doi: 10.11648/j.sjc.20221004.14

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@article{10.11648/j.sjc.20221004.14,
  author = {Papa Biram Sy and Aissatou Alioune Gaye and Alioune Fall},
  title = {Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves},
  journal = {Science Journal of Chemistry},
  volume = {10},
  number = {4},
  pages = {120-125},
  doi = {10.11648/j.sjc.20221004.14},
  url = {https://doi.org/10.11648/j.sjc.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20221004.14},
  abstract = {The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.},
 year = {2022}
}

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TY  - JOUR
T1  - Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves
AU  - Papa Biram Sy
AU  - Aissatou Alioune Gaye
AU  - Alioune Fall
Y1  - 2022/08/29
PY  - 2022
N1  - https://doi.org/10.11648/j.sjc.20221004.14
DO  - 10.11648/j.sjc.20221004.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 120
EP  - 125
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20221004.14
AB  - The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.
VL  - 10
IS  - 4
ER  -

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Author Information

Papa Biram Sy

Department of Chemistry, University Cheikh Anta Diop, Dakar, Senegal
Aissatou Alioune Gaye

Center for Food Security and Functional Molecules Studies, University Cheikh Anta Diop, Dakar, Senegal
Alioune Fall

Department of Chemistry, University Cheikh Anta Diop, Dakar, Senegal

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Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. (2022). Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Science Journal of Chemistry, 10(4), 120-125. https://doi.org/10.11648/j.sjc.20221004.14

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ACS Style

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci. J. Chem. 2022, 10(4), 120-125. doi: 10.11648/j.sjc.20221004.14

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AMA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci J Chem. 2022;10(4):120-125. doi: 10.11648/j.sjc.20221004.14

Copy | Download

@article{10.11648/j.sjc.20221004.14,
  author = {Papa Biram Sy and Aissatou Alioune Gaye and Alioune Fall},
  title = {Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves},
  journal = {Science Journal of Chemistry},
  volume = {10},
  number = {4},
  pages = {120-125},
  doi = {10.11648/j.sjc.20221004.14},
  url = {https://doi.org/10.11648/j.sjc.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20221004.14},
  abstract = {The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.},
 year = {2022}
}

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TY  - JOUR
T1  - Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves
AU  - Papa Biram Sy
AU  - Aissatou Alioune Gaye
AU  - Alioune Fall
Y1  - 2022/08/29
PY  - 2022
N1  - https://doi.org/10.11648/j.sjc.20221004.14
DO  - 10.11648/j.sjc.20221004.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 120
EP  - 125
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20221004.14
AB  - The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.
VL  - 10
IS  - 4
ER  -

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