Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou

doi:doi:10.11648/j.sjph.20210901.11

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Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou

Published in Science Journal of Public Health (Volume 9, Issue 1)

Received: 18 December 2020 Published: 18 January 2021

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Abstract

Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.

Published in	Science Journal of Public Health (Volume 9, Issue 1)
DOI	10.11648/j.sjph.20210901.11
Page(s)	1-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Iodine Nutritional Status, Graves' Disease, Th17 Cells and Treg cells, Cytokines and Transcription Factors

References

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APA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. (2021). Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Science Journal of Public Health, 9(1), 1-11. https://doi.org/10.11648/j.sjph.20210901.11

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ACS Style

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(1), 1-11. doi: 10.11648/j.sjph.20210901.11

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AMA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(1):1-11. doi: 10.11648/j.sjph.20210901.11

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@article{10.11648/j.sjph.20210901.11,
  author = {Jing Feng and Cuicui Wang and Zhaoxin Mu and Xinsheng Li and Zhenjiang Hou},
  title = {Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.sjph.20210901.11},
  url = {https://doi.org/10.11648/j.sjph.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.11},
  abstract = {Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.},
 year = {2021}
}

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TY  - JOUR
T1  - Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status
AU  - Jing Feng
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Xinsheng Li
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.11
DO  - 10.11648/j.sjph.20210901.11
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.11
AB  - Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.
VL  - 9
IS  - 1
ER  -

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Author Information

Jing Feng

Cangzhou Central Hospital, Cangzhou, China
Cuicui Wang

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Zhaoxin Mu

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Xinsheng Li

Cangzhou Central Hospital, Cangzhou, China
Zhenjiang Hou

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China

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APA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. (2021). Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Science Journal of Public Health, 9(1), 1-11. https://doi.org/10.11648/j.sjph.20210901.11

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ACS Style

Jing Feng; Cuicui Wang; Zhaoxin Mu; Xinsheng Li; Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(1), 1-11. doi: 10.11648/j.sjph.20210901.11

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AMA Style

Jing Feng, Cuicui Wang, Zhaoxin Mu, Xinsheng Li, Zhenjiang Hou. Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(1):1-11. doi: 10.11648/j.sjph.20210901.11

Copy | Download

@article{10.11648/j.sjph.20210901.11,
  author = {Jing Feng and Cuicui Wang and Zhaoxin Mu and Xinsheng Li and Zhenjiang Hou},
  title = {Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.sjph.20210901.11},
  url = {https://doi.org/10.11648/j.sjph.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.11},
  abstract = {Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Research on the Role of Th17Treg Cells and Their Factors in Graves' Disease with Different Iodine Nutritional Status
AU  - Jing Feng
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Xinsheng Li
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.11
DO  - 10.11648/j.sjph.20210901.11
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.11
AB  - Introduction: At present, studies on the role of iodine nutrition in thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of GD have not been carried out. Objective: The acritical aims to investigate the correlation between thyroid function and autoantibody titers of Graves' disease (GD) patients with different iodine nutritional status with Th17/Treg cells, their cytokines and transcription factors, and the role of related factors in the pathogenesis of GD. Method: The levels of serum thyroid hormone, autoantibodies and urine iodine in 100 GD patients and 60 healthy subjects are detected by electrochemiluminescence instrument and iodine-catalyzed arsenic-cerium method, respectively. The ratio of Th17 cells to Treg cells and Th17/Treg ratio in peripheral blood mononuclear cells (PBMC) are detected by immunofluorescence-labeled monoclonal antibodies and flow cytometry. Real-time fluorescence quantitative PCR is used to detect the expression levels of retinoic acid-related orphan receptor (ROR-γt) and fork head/wing-shaped spiral transcription factor 3 (Foxp3) mRNA, and the serum IL-17 and TGF-β levels are detected by ELISA. Result: As a result, the proportion of Th17 cells, serum IL-17 and ROR-γt in PBMC of GD patients with different iodine nutritional status significantly increase, while the proportion of Treg cells, the expression of Foxp3mRNA and serum TGF-β significantly decrease. The ratio of Th17/Treg cells in GD patients is significantly positively correlated with the titers of TPOAb and TgAb, and the titers of TPOAb and TgAb antibodies are significantly correlated with Th17/Treg, IL-17 and ROR-γt. Conclusion: In conclusion, thyroid hormones, autoantibodies, Th17, Treg cell ratios and dysfunctions as well as corresponding cytokines and transcription factors in GD patients with different iodine nutritional status participate in the development of GD.
VL  - 9
IS  - 1
ER  -

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