The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice

Torricelli P.; Ferorelli P.; De Martino A.; Antonelli F.; Beninati S.

doi:doi:10.11648/j.ajls.20130102.16

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The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice

Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S.

Published in American Journal of Life Sciences (Volume 1, Issue 2)

Received: 12 May 2013 Published: 30 May 2013

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Abstract

Liver steatosis development was obtained after high cholesterol diet (HCD) in C57BL6/N mice. Mice were preliminarily treated for 10 days with multiple micronutrients contained in a commercial food supplement called Citozym (CIZ), and successively for 40 days with CIZ and HCD, to compare the protection effect of used compounds on liver metabolism and metabolic steatosis. At the end of the experiments, livers were dissected for histological examinations. Plasma total cholesterol (TCH), triacylglycerol (TAG) aspartate aminotransferase (AST), and alanine aminotransferase (ALT) concentrations were significantly higher in all HCD-fed mice. These data support a lipotoxic model of cholesterol-mediated hepatic steatosis. We found that the administration of CIZ to HCD-mouse model of steatosis significantly decreased plasma TCH, TAG, ALT and AST levels along with a reduction of the accumulated fat and inflammation in the liver. These findings suggest that a preventive and continuative treatment of CIZ exerts a protective effect against metabolic hepatosteatosis.

Published in	American Journal of Life Sciences (Volume 1, Issue 2)
DOI	10.11648/j.ajls.20130102.16
Page(s)	55-60
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Micronutrients, Antioxidants, Cholesterol, NAFLD, Metabolic Steatosis

References

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Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S. (2013). The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. American Journal of Life Sciences, 1(2), 55-60. https://doi.org/10.11648/j.ajls.20130102.16

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ACS Style

Torricelli P.; Ferorelli P.; De Martino A.; Antonelli F.; Beninati S. The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. Am. J. Life Sci. 2013, 1(2), 55-60. doi: 10.11648/j.ajls.20130102.16

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AMA Style

Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S. The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. Am J Life Sci. 2013;1(2):55-60. doi: 10.11648/j.ajls.20130102.16

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@article{10.11648/j.ajls.20130102.16,
  author = {Torricelli P. and Ferorelli P. and De Martino A. and Antonelli F. and Beninati S.},
  title = {The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {2},
  pages = {55-60},
  doi = {10.11648/j.ajls.20130102.16},
  url = {https://doi.org/10.11648/j.ajls.20130102.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130102.16},
  abstract = {Liver steatosis development was obtained after high cholesterol diet (HCD) in C57BL6/N mice. Mice were preliminarily treated for 10 days with multiple micronutrients contained in a commercial food supplement called Citozym (CIZ), and successively for 40 days with CIZ and HCD, to compare the protection effect of used compounds on liver metabolism and metabolic steatosis. At the end of the experiments, livers were dissected for histological examinations. Plasma total cholesterol (TCH), triacylglycerol (TAG) aspartate aminotransferase (AST), and alanine aminotransferase (ALT) concentrations were significantly higher in all HCD-fed mice. These data support a lipotoxic model of cholesterol-mediated hepatic steatosis. We found that the administration of CIZ to HCD-mouse model of steatosis significantly decreased plasma TCH, TAG, ALT and AST levels along with a reduction of the accumulated fat and inflammation in the liver. These findings suggest that a preventive and continuative treatment of CIZ exerts a protective effect against metabolic hepatosteatosis.},
 year = {2013}
}

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TY - JOUR
T1 - The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice
AU - Torricelli P.
AU - Ferorelli P.
AU - De Martino A.
AU - Antonelli F.
AU - Beninati S.
Y1 - 2013/05/30
PY - 2013
N1 - https://doi.org/10.11648/j.ajls.20130102.16
DO - 10.11648/j.ajls.20130102.16
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 55
EP - 60
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20130102.16
AB - Liver steatosis development was obtained after high cholesterol diet (HCD) in C57BL6/N mice. Mice were preliminarily treated for 10 days with multiple micronutrients contained in a commercial food supplement called Citozym (CIZ), and successively for 40 days with CIZ and HCD, to compare the protection effect of used compounds on liver metabolism and metabolic steatosis. At the end of the experiments, livers were dissected for histological examinations. Plasma total cholesterol (TCH), triacylglycerol (TAG) aspartate aminotransferase (AST), and alanine aminotransferase (ALT) concentrations were significantly higher in all HCD-fed mice. These data support a lipotoxic model of cholesterol-mediated hepatic steatosis. We found that the administration of CIZ to HCD-mouse model of steatosis significantly decreased plasma TCH, TAG, ALT and AST levels along with a reduction of the accumulated fat and inflammation in the liver. These findings suggest that a preventive and continuative treatment of CIZ exerts a protective effect against metabolic hepatosteatosis.
VL - 1
IS - 2
ER -

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Author Information

Torricelli P.

Department SPES, University of Molise, Campobasso, Italy
Ferorelli P.

Department SPES, University of Molise, Campobasso, Italy
De Martino A.

University of Rome, Tor Vergata, Dept Biology, Roma, Italy
Antonelli F.

University of Rome, Tor Vergata, Dept Biology, Roma, Italy
Beninati S.

University of Rome, Tor Vergata, Dept Biology, Roma, Italy

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APA Style

Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S. (2013). The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. American Journal of Life Sciences, 1(2), 55-60. https://doi.org/10.11648/j.ajls.20130102.16

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ACS Style

Torricelli P.; Ferorelli P.; De Martino A.; Antonelli F.; Beninati S. The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. Am. J. Life Sci. 2013, 1(2), 55-60. doi: 10.11648/j.ajls.20130102.16

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AMA Style

Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S. The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice. Am J Life Sci. 2013;1(2):55-60. doi: 10.11648/j.ajls.20130102.16

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@article{10.11648/j.ajls.20130102.16,
  author = {Torricelli P. and Ferorelli P. and De Martino A. and Antonelli F. and Beninati S.},
  title = {The Influence of Preventive Multiple Micronutrients Supplementation on Liver Steatosis in High-cholesterol Fed C57BL6/N Mice},
  journal = {American Journal of Life Sciences},
  volume = {1},
  number = {2},
  pages = {55-60},
  doi = {10.11648/j.ajls.20130102.16},
  url = {https://doi.org/10.11648/j.ajls.20130102.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130102.16},
  abstract = {Liver steatosis development was obtained after high cholesterol diet (HCD) in C57BL6/N mice. Mice were preliminarily treated for 10 days with multiple micronutrients contained in a commercial food supplement called Citozym (CIZ), and successively for 40 days with CIZ and HCD, to compare the protection effect of used compounds on liver metabolism and metabolic steatosis. At the end of the experiments, livers were dissected for histological examinations. Plasma total cholesterol (TCH), triacylglycerol (TAG) aspartate aminotransferase (AST), and alanine aminotransferase (ALT) concentrations were significantly higher in all HCD-fed mice. These data support a lipotoxic model of cholesterol-mediated hepatic steatosis. We found that the administration of CIZ to HCD-mouse model of steatosis significantly decreased plasma TCH, TAG, ALT and AST levels along with a reduction of the accumulated fat and inflammation in the liver. These findings suggest that a preventive and continuative treatment of CIZ exerts a protective effect against metabolic hepatosteatosis.},
 year = {2013}
}

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