Polyphosphate accumulating organisms were isolated from water and sludge samples of intensive catfish ponds in the Mekong Delta, Vietnam. The Results of estimation of intracellular polyphosphate concentration conducted on each of monocultures indicated that the content of intracellular polyphosphate varied from 2 mg/l to 148.1 mg/l after 6 days of incubation in the medium. Of 191 isolates, twenty-one have uptake and store intracellular phosphate from 19.6 to 148.1 mg/l. They have shaped like a rods and short rods or cocci, a few of them were slightly curved or straight or curved rods. The majority of them are gram-positive (76.2%) and the remains are gram-negative. The partial 16S rRNA genes of these isolates were sequenced and compared with bacterial 16S rRNA genes in Genbank using BlastN Program. Phylogenetic tree was constructed on the basic 16S rRNA gene sequences demonstrating the population of high phosphate accumulating bacteria obtained from samples of catfish ponds were affiliated with four major bacterial lineages. Twenty-one bacteria isolates from samples of catfish ponds included in four classes: Bacilli, Actinobacteria, Beta-proteobacteria, Gamma-proteobacteria. The majority of the strains showed excess phosphate accumulation. Strains related to Bacillus sp. were dominant bacteria group constituted up to 52.4% of all identified isolates, but high phosphate accumulating bacteria are Burkholderia vietnamiensis TVT003L within class Beta-proteobacteria, Acinetobacter radioresistens TGT013L within Gamma-proteobacteria and Arthrobacter protophomiae VLT002L within class Actinobacteria. Methyl blue Loeffler’s staning and electron microscopy examination confirmed that the bacteria had stored polyphosphate granules intracellularly.
| Published in | American Journal of Life Sciences (Volume 1, Issue 2) |
| DOI | 10.11648/j.ajls.20130102.17 |
| Page(s) | 61-71 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2013. Published by Science Publishing Group |
Polyphosphate Accumulating Organisms, Polyphosphate, Phosphate Removal, Catfish Pond, Bacilli, Actinobacteria
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APA Style
Le Quang Khoi, Cao Ngoc Diep. (2013). Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms in Water and Sludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam. American Journal of Life Sciences, 1(2), 61-71. https://doi.org/10.11648/j.ajls.20130102.17
ACS Style
Le Quang Khoi; Cao Ngoc Diep. Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms in Water and Sludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam. Am. J. Life Sci. 2013, 1(2), 61-71. doi: 10.11648/j.ajls.20130102.17
AMA Style
Le Quang Khoi, Cao Ngoc Diep. Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms in Water and Sludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam. Am J Life Sci. 2013;1(2):61-71. doi: 10.11648/j.ajls.20130102.17
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Download@article{10.11648/j.ajls.20130102.17,
author = {Le Quang Khoi and Cao Ngoc Diep},
title = {Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms in Water and Sludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam},
journal = {American Journal of Life Sciences},
volume = {1},
number = {2},
pages = {61-71},
doi = {10.11648/j.ajls.20130102.17},
url = {https://doi.org/10.11648/j.ajls.20130102.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130102.17},
abstract = {Polyphosphate accumulating organisms were isolated from water and sludge samples of intensive catfish ponds in the Mekong Delta, Vietnam. The Results of estimation of intracellular polyphosphate concentration conducted on each of monocultures indicated that the content of intracellular polyphosphate varied from 2 mg/l to 148.1 mg/l after 6 days of incubation in the medium. Of 191 isolates, twenty-one have uptake and store intracellular phosphate from 19.6 to 148.1 mg/l. They have shaped like a rods and short rods or cocci, a few of them were slightly curved or straight or curved rods. The majority of them are gram-positive (76.2%) and the remains are gram-negative. The partial 16S rRNA genes of these isolates were sequenced and compared with bacterial 16S rRNA genes in Genbank using BlastN Program. Phylogenetic tree was constructed on the basic 16S rRNA gene sequences demonstrating the population of high phosphate accumulating bacteria obtained from samples of catfish ponds were affiliated with four major bacterial lineages. Twenty-one bacteria isolates from samples of catfish ponds included in four classes: Bacilli, Actinobacteria, Beta-proteobacteria, Gamma-proteobacteria. The majority of the strains showed excess phosphate accumulation. Strains related to Bacillus sp. were dominant bacteria group constituted up to 52.4% of all identified isolates, but high phosphate accumulating bacteria are Burkholderia vietnamiensis TVT003L within class Beta-proteobacteria, Acinetobacter radioresistens TGT013L within Gamma-proteobacteria and Arthrobacter protophomiae VLT002L within class Actinobacteria. Methyl blue Loeffler’s staning and electron microscopy examination confirmed that the bacteria had stored polyphosphate granules intracellularly.},
year = {2013}
}
TY - JOUR T1 - Isolation and Phylogenetic Analysis of Polyphosphate Accumulating Organisms in Water and Sludge of Intensive Catfish Ponds in the Mekong Delta, Vietnam AU - Le Quang Khoi AU - Cao Ngoc Diep Y1 - 2013/05/30 PY - 2013 N1 - https://doi.org/10.11648/j.ajls.20130102.17 DO - 10.11648/j.ajls.20130102.17 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 61 EP - 71 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20130102.17 AB - Polyphosphate accumulating organisms were isolated from water and sludge samples of intensive catfish ponds in the Mekong Delta, Vietnam. The Results of estimation of intracellular polyphosphate concentration conducted on each of monocultures indicated that the content of intracellular polyphosphate varied from 2 mg/l to 148.1 mg/l after 6 days of incubation in the medium. Of 191 isolates, twenty-one have uptake and store intracellular phosphate from 19.6 to 148.1 mg/l. They have shaped like a rods and short rods or cocci, a few of them were slightly curved or straight or curved rods. The majority of them are gram-positive (76.2%) and the remains are gram-negative. The partial 16S rRNA genes of these isolates were sequenced and compared with bacterial 16S rRNA genes in Genbank using BlastN Program. Phylogenetic tree was constructed on the basic 16S rRNA gene sequences demonstrating the population of high phosphate accumulating bacteria obtained from samples of catfish ponds were affiliated with four major bacterial lineages. Twenty-one bacteria isolates from samples of catfish ponds included in four classes: Bacilli, Actinobacteria, Beta-proteobacteria, Gamma-proteobacteria. The majority of the strains showed excess phosphate accumulation. Strains related to Bacillus sp. were dominant bacteria group constituted up to 52.4% of all identified isolates, but high phosphate accumulating bacteria are Burkholderia vietnamiensis TVT003L within class Beta-proteobacteria, Acinetobacter radioresistens TGT013L within Gamma-proteobacteria and Arthrobacter protophomiae VLT002L within class Actinobacteria. Methyl blue Loeffler’s staning and electron microscopy examination confirmed that the bacteria had stored polyphosphate granules intracellularly. VL - 1 IS - 2 ER -
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